hERG safety testing

hERG Screening & Cardiac Ion Channel Profiling

hERG safety testing

 In vitro pharmacological profiling of cardiac ion channels at an early stage in the discovery process helps alert researchers to unwanted modulation of ion channels involved in excitation and relaxation of cardiac muscle. This in turn aids decision-making in lead generation, lead optimization and candidate selection, resulting in significantly reduced downstream safety-related attrition.    

SB Drug Discovery offers comprehensive cardiac ion channel safety profiling using conventional patch clamp and automated high throughput electrophysiology. Choose from stand-alone hERG screening & profiling or select either our core cardiac panel encompassing hERG, Cav1.2 and Nav 1.5 or our extended panel which also includes Kir 2.1, Kv4.3, KCNQ1, HCN4 and Kv 1.5.


hERG Screening

hERG profiling assay

hERG Profiling: Representative hERG electrophysiology current trace showing control current (grey), 30 nM cisapride (blue) and full block (black).

 

hERG profiling assay

hERG Profiling: Representative hERG time-course showing control, 30 nM cisapride (blue) and full block with 10 uM cisapride.


 

Cav 1.2 Screening

Cav1.2 screening assay

Cav 1.2 Profiling: Representative Cav 1.2 electrophysiology current trace showing control current (grey), 100 nM nifedipine (blue) and full block (black).

 

 

Cav1.2 profiling

Cav 1.2 Profiling: Representative Cav 1.2 time-course showing control, 100 nM nifedipine (blue) and nifedipine full-block.


 

Nav1.5 Screening

Nav1.5 profiling

Nav 1.5 Profiling: Representative Nav 1.5 electrophysiology current trace showing control current (grey), 1uM propafenone (blue) and full block (black).

 

 

Image

Nav 1.5 Profiling: Representative Nav 1.5 time-course showing control, 1uM propafenone (blue) followed by full-block.


Cardiac Safety Panel

hERG Cav 1.2 Nav 1.5
Kv4.3 KvLQT1 Kir2.1
Kv1.5 HCN4  

To discuss your next ion channel project or to receive a quote, contact us or call +44 141 587 6100.


Ion channel cell lines
Ion channel screening