Cell Line Development
Customized cell line generation designed to meet your screening needs.
Stable cell lines expressing recombinant targets such as ion channels and GPCRs are commonly used in a number of research applications including drug discovery screening and safety assessment. SB has overseen generation of more than 200 functionally validated custom cell lines with particular focus on over-expression of membrane proteins. Our cell line generation services can be tailored to your needs including choice of host cell, constitutive or inducible expression and customized validation.
SB specializes not only in generating cell lines expressing human targets but also in species variants and disease related mutants. Our extensive experience has provided invaluable understanding of the complexities faced in generation of such cell lines.
Ion channel targets
Extensive expertise in ion channel cell line generation across a broad range of channel types. Stable clones are screened using FLIPR and / or SyncroPatch to identify those clones showing optimal responses and accurate pharmacology.
Broad range of GPCR screening assays to identify optimal clones. SB utilizes FACS, ligand binding, calcium mobilization and cAMP assays to ensure optimal GPCR clones are selected with accurate pharmacology.
Inducible expression: The challenge of expressing proteins which cause a toxic effect as a result of long term expression can be overcome with the use of inducible expression systems.
Species orthologs: Cell lines expressing species orthologs are key tools in early stage drug discovery prior to entering into in vivo studies. SB’s has expertise in generating novel species cDNA from limited starting information