Ion Channel Studies
TMEM175
Access the TMEM175 Assays You Need
Sygnature Discovery offers a suite of proprietary cell lines expressing TMEM175, supporting both wild-type and variant forms as well as key species orthologs.
Our validated cell models and robust electrophysiology platforms are designed to help you:
- Accelerate hit identification and lead optimization
- Explore functional effects of disease-relevant TMEM175 variants
- Confidently advance TMEM175-targeting drug discovery programs
Need something specific? Our experience cell line generation team is also on hand to develop and validate additional TMEM175 variants to support your research.
| Off-the-Shelf TMEM175 Assays | ||
|---|---|---|
| TMEM175 (human) | TMEM175 (LoF) | TMEM175 (GoF) |
| TMEM175 (mouse) | TMEM175 (rat) | TMEM175 (dog) |
| TMEM175 (monkey) | ||
Investigate TMEM175 Biology in Depth
Our TMEM175 platform allows researchers to:
- Assess differential activation in response to extracellular acidification
- Evaluate variant-specific pharmacological responses
- Measure compound efficacy across disease-relevant genetic backgrounds
With side-by-side comparisons of wild-type, LoF and GoF TMEM175 variants, you can better understand how genetic diversity impacts TMEM175 activity – and discover new ways to target it.
Contact Syganture Discovery
To learn more about our services or to request a quote.
TMEM175 screening assays
Validated high-throughput screening assay
Robust electrophysiology assays ready to run
Disease-relevant variant cell lines & assays
Range of species orthologs
Validated High-Throughput Screening
Sygnature Discovery has deployed its TMEM175 screening cascade to successfully identify a diverse range of modulators with clear, concentration-dependent effect.
Our success supports the platform’s ability to:
- Detect subtle functional changes across wild-type, LoF, and GoF variants
- Quantify modulator activity with robust EC₅₀/IC₅₀ curves
- Assess variant-specific activity profiles to support disease relevance
This validated screening platform enables the discovery of novel pharmacological tools to modulate TMEM175 — advancing efforts to restore lysosomal homeostasis and better understand the channel’s role in neurodegenerative disease mechanisms.
Figure 1: Representative TMEM175 current traces before (grey line) and during the presence of increasing concentrations of the agonist DCPIB (blue lines). Currents are evoked by a 500 ms ramp from -80 mV to +80mV, followed by a 500 ms step from -80 mV to +80 mV before returning to the holding potential of 0mV.
SSM Electrophysiology for Lysosomal Ion Channels
Complementing our automated patch clamp capabilities, Sygnature Discovery also offers solid-supported membrane (SSM)-based electrophysiology – an ideal approach for studying intracellular lysosomal ion channels such as TMEM175.
- Reconstituted lysosomal membranes containing TMEM175
- Both activators and inhibitors can be evaluated in high-throughput 96-well format
- Orthogonal validation to support HTS findings and confirm organellar ion channel modulation
Figure 2: Representative TMEM175 current traces measured in the presence of increasing concentrations of the agonist DCPIB (0.3-30 µM) using solid-supported membrane (SSM) electrophysiology platform.
Accelerate Your TMEM175 Discovery Program
Whether you are screening novel modulators, developing a fast-follower approach or characterizing effects on TMEM175 variants, Sygnature Discovery’s TMEM175 platform offers expertise, flexibility and quality to support your discovery needs.
Contact Syganture Discovery
To learn more about our services or to request a quote.
Case Study
A Multi-Platform Approach to TMEM175 Drug Discovery
TMEM175 is a novel, constitutively active ion channel involved in the regulation of lysosomal pH, lysosomal Δψ and autophagy. TMEM175’s significance lies not only in its physiological role but also in its therapeutic promise.
